Responsive Emulsions for Sequential Multienzyme Cascades

Multienzyme cascade biocatalysis is an efficient synthetic process, avoiding the isolation/purification of intermediates and shifting reaction equilibrium to product side.. However, multienzyme systems are often limited by their incompatibility cross-reactivity. Herein, we report a multi-responsive emulsion proceed reactions sequentially for high reactivity. The achieved using CO2, pH, thermo-responsive block copolymer as stabilizer, allowing on-demand control morphology phase composition. Applying this system three-step enables individual optimal condition each enzyme, overall conversion (ca. 97 % calculated limit) thereby obtained. Moreover, multi-responsiveness allows facile separate yielding/recycling products, polymers active enzymes. Besides, could be scaled up with good yield.